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Plant Biology Faculty Plant Biology Home Genetics Faculty by Area Genetics Adjunct and Associated Faculty ... Genetics Home Regulation of Embryo Development Glenn Galau Associate Professor BA - Biology, Lawrence University, 1971 PhD - Developmental Biology, Caltech, 1976 Department of Plant Biology Plant Sciences Building University of Georgia Athens, GA 30602-7271 USA Wayne Hughes Senior Research Associate BS - Chemistry, Florida State University, 1977 PhD - Analytical Chemistry, University of Georgia, 1983 Department of Plant Biology Plant Sciences Building University of Georgia Athens, GA 30602-7271 USA Undergraduate Research Abstracts Instruction Developmental Programs of Gene Activity The accumulation kinetics of 50 cloned cotton mRNA markers define several sequential developmental programs in embryos. These are associated with the cotyledon-stage, the concentration of endogenous abscisic acid, the maturation or reserve accumulation stage, ovule abscission, and germination/early seedling growth. Gene expression is temporally modular (combinatorial), suggesting that genes that are expressed in more than one program are regulated by program-specific factors rather than by factors that are common to several programs. Both descriptive and experimental studies indicate that ovule abscission terminates the maturation program and induces the postabscission program while imbibition induces the germination/early seedling growth program. Desiccation Protection The structures of many of the postabscission-specific LEA proteins suggest that a major function of the embryo postabscission program, in which they are expressed, is to prepare the embryo for desiccation. Their expression is consistent with this notion; the postabscission program can be induced in immature or germinating embryos by abscisic acid, osmotic stress or desiccation. One of the approaches that we are using to address the regulation and function of these genes is to study embryo desiccation-lethal mutants in

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