141. PROTOCOLS FOR RECOMBINANT DNA ISOLATION, CLONING, And SEQUENCING PROTOCOLS FOR RECOMBINANT DNA ISOLATION, cloning, and SEQUENCING. 1. Sambrook, J., Fritsch, EF, and Maniatis, T., in Molecular cloning A Laboratory Manual. http://www.genome.ou.edu/protocol_book/protocol_index.html

PROTOCOLS FOR RECOMBINANT DNA ISOLATION, CLONING, and SEQUENCING edited by Bruce A. Roe Judy S. Crabtree and Akbar S. Khan Department of Chemistry and Biochemistry The University of Oklahoma Norman, Oklahoma 73019 e-mail: broe@ou.edu phone: 405 325-4912 fax: 405 325-7762 August 5, 1995 A printed version of this protocol book can be obtained from: Introduction This manual is a compilation of many of the everyday methods used in the average molecular biology laboratory, with emphasis on the techniques for large scale DNA sequencing protocols and DNA sequencing automation techniques. The manual has been written in a protocol format, with little theoretical discussion. For theory and additional information, users of this manual are referred back to the original literature, or to other textual manuals such as those published by Maniatis (1) et al. and Glover (2). The following persons are acknowledged for contributing methods and suggestions during the assembly of this manual: Stephanie Chissoe, Sandy Clifton, Dennis Burian, Rick Wilson, Din-Pow Ma, James Wong, Leslie Johnston-Dow, Elaine Mardis, Zhili Wang, Kala Iyer, Steve Toth, Goughay Zhang, Hua Qin Pan and other members of the Roe laboratory, both past and present.

142. CNN.com - Academy Of Sciences Urges Ban On Human Cloning - January 18, 2002 CNN http://cnn.com/2002/HEALTH/01/18/academies.cloning/index.html

MAIN PAGE WORLD U.S. WEATHER ... ABOUT US/HELP CNN TV what's on show transcripts CNN Headline News CNN International ... askCNN EDITIONS CNN.com Asia CNN.com Europe CNNenEspanol.com CNNArabic.com ... set your edition Languages Spanish Portuguese German Italian Korean Arabic Japanese Time, Inc. Time.com People Fortune EW InStyle Business 2.0 Academy of Sciences urges ban on human cloning Dr. Irving Weissman chaired the panel that looked at the safety of human cloning. (CNN) The National Academy of Sciences recommended Friday that human reproductive cloning cloning to create a baby be legally banned. "Human reproductive cloning should not now be practiced. It is dangerous and likely to fail," Dr. Irving Weissman, the chairman of the panel that made the recommendation, said while presenting the findings at a news conference. Despite these misgivings, the panel said the issue of human reproductive cloning should be revisited in five years if a medical and scientific review suggests techniques may be safer, and if there is a public consensus that a review is warranted. While the panel called for human cloning to be banned, it said that ban should not extend to the nuclear transfer technique, or cloning embryos for the purpose of extracting stem cells for the treatment of disease, "because of its considerable potential for developing new medical therapies for life-threatening diseases."

143. CNN.com - Group Says It Will Move Human Cloning Work Offshore - June 29, 2001 CNN http://cnn.com/2001/HEALTH/06/29/cloning.lab/index.html

MAIN PAGE WORLD U.S. WEATHER ... ABOUT US CNN TV what's on show transcripts CNN Headline News CNN International ... askCNN EDITIONS CNN.com Asia CNN.com Europe set your edition Languages Time, Inc. Time.com People Fortune EW Group says it will move human cloning work offshore Boisselier says she hopes to successfully clone a human within a year By Miriam Falco CNN Medical Producer (CNN) The Food and Drug Administration is cracking down on a religous group's attempts to clone a human being, a researcher said Friday. Brigitte Boisselier, the chief scientist for a company called Clonaid which was founded by a religious group called the Raelians said FDA investigators visited her laboratory in the spring and told her to stop her experiments. Boisselier said the Raelians, who believe human life on Earth was the result of genetic experiments by extraterrestrials, will move their work to another country. "I will never do anything illegal, that is why I decided to move part of the lab to another country," she said. She would not disclose the laboratory's current location. MORE STORIES House hearings turn skeptical eye on cloning U.S. lawmakers debate limits on human cloning

144. Frontline: Making Babies: Human Cloning You predicted, I think two years ago, that human cloning would be here with us, within two years. I m not advocating the use of cloning in this way. http://www.pbs.org/wgbh/pages/frontline/shows/fertility/etc/cloning.html

var loc = "../../../"; Silver is a Professor of Genetics at Princeton University where his laboratory is attempting to identify genes that influence personality and behavior. You predicted, I think two years ago, that human cloning would be here with us, within two years. I don't think I said that ... I predicted that human cloning would be with us in 10 years and I still believe that is the case, because there is a demand among a small number of people for this technology to have babies. It's being driven by the marketplace. I think that, ethically, one should not use this technology until they are convinced that it is safe and efficient, shown with the use of animals. But I don't think that physicians around the world are going to wait for the confirmation that it's safe and efficient in animals. The best example I can give you why physicians are not going to wait as they should is with ICSI, an intracytoplasmic sperm injection. This was a new technology developed in the early 1990s to overcome severe infertility and physicians did not wait to prove that it wasn't going to cause birth defects before they embraced it wholly across the country. We can use that history to understand how cloning is going to go. I'm not advocating the use of cloning in this way. I think it is wrong, but it's going to happen. Can you explain simply what cloning is, because [some] people think that it's the creation of an adult copy.

145. CNN.com - U.S. Cloning Advance Shocks World - November 26, 2001 CNN http://cnn.com/2001/TECH/science/11/26/human.cloning.reax1200/index.html

MAIN PAGE WORLD U.S. WEATHER ... ABOUT US/HELP CNN TV what's on show transcripts CNN Headline News CNN International ... askCNN EDITIONS CNN.com Asia CNN.com Europe CNNenEspanol.com CNNArabic.com ... set your edition Languages Spanish Portuguese German Italian Korean Arabic Japanese Time, Inc. Time.com People Fortune EW InStyle Business 2.0 U.S. cloning advance shocks world LONDON, England Political and religious leaders around the world have condemned the latest breakthrough in cloning research in which a U.S. company said it had cloned a human embryo for the first time. The private U.S. research company Advanced Cell Technology (ACT), based in Worcester, Massachusetts said on Sunday it had cloned embryos by removing the DNA from human egg cells. The DNA from an adult human body cell was then implanted into the egg cell, which was then stimulated to grow into a six-cell embryo. VIDEO President George W. Bush speaks out against cloning of human beings. CNN's Kelly Wallace reports (November 26) Play video (QuickTime, Real or Windows Media) MORE STORIES The company behind the clones: Advanced Cell Technology CNN Access: "I'm just trying to help people who are sick"

146. NOVA Online | 18 Ways To Make A Baby | On Human Cloning On Human cloning Three Views The birth of Dolly, the first mammal cloned from the cell of an adult animal, sent intellectual and emotional shockwaves around http://www.pbs.org/wgbh/nova/baby/cloning.html

The sheep that shook the world. On Human Cloning: Three Views The birth of Dolly, the first mammal cloned from the cell of an adult animal, sent intellectual and emotional shockwaves around the world when it was reported in early 1997. What's next? commentators asked. Could human beings now begin making carbon copies of themselves? If so, will those with the means use cloning to essentially cheat mortality? Could a form of the eugenics espoused by the Nazis now become reality if, say, a rogue government so chose? The cloning of human beings, many concluded, would be biologically wrong, socially misguided, and morally and ethically repugnant. Yet some scientists began touting the enormous benefits that human cloning might bring. These include helping infertile couples who have had no luck with other infertility treatments to have children or allowing a parent bearing a gene for a debilitating disease such as Huntington's chorea to avoid passing the gene onto his or her child. In theory, specialists could also use cloning to grow embryonic stem cells into vital organs, blood, or tissue, which doctors could then use for transplants, transfusions, and other replacement interventions. Here we present three points of view on this highly contentious issue. All three scientists are experts on the subject who all have the same facts at their disposal. Yet Dr. Lee Silver, a Princeton molecular biologist, remains bullish on the idea of cloning humans, while Dr. Rudolf Jaenisch, professor of biology at MIT, just as adamantly opposes the idea, under any circumstances. Dr. Don Wolf, a senior scientist at the Oregon Regional Primate Research Center, falls somewhere in between that is, he has serious reservations but is not opposed in principle. Read these interviews and decide for yourself.

147. CNN.com - Genetic Discovery Has Implications For Cloning, Cancer - August 9, 200 CNN http://cnn.com/2001/HEALTH/08/09/dna.switch/index.html

MAIN PAGE WORLD U.S. WEATHER ... ABOUT US CNN TV what's on show transcripts CNN Headline News CNN International ... askCNN EDITIONS CNN.com Asia CNN.com Europe set your edition Languages Time, Inc. Time.com People Fortune EW Genetic discovery has implications for cloning, cancer NEW YORK (CNN) A newly discovered genetic process offers tantalizing clues for cancer researchers and reveals possible obstacles to cloning, scientists report. Results show that despite the success of mapping the human genetic code the human genome it is only a first step in understanding how genes work. Four articles in this week's Science magazine detail how genes in human DNA appear to be controlled by a sea of surrounding proteins that functions as a master switch. Molecular geneticists call the newly discovered process the 'histone code' and say they are just beginning to translate it. The articles describe methylation, a chemical process that acts as a trigger. Scientists have watched the common organic molecule methyl interact with the proteins surrounding DNA, and say the cell appears to use the process to switch blocks of genes on and off. The discovery may be a new argument against human cloning. Even with the right cell and the right DNA, until scientists understand "this parallel rule book of genetics," they cannot clone without error, said researcher C. Davis Allis at the University of Virginia, who worked on the projects.

148. American Bioethics Advisory Commission cloning. cloning When word games kill. Kevin Fitzgerald. Other cloning articles GENETICIST Jerome Lejeune s observations about cloning. http://www.all.org/abac/cloning.htm

Cloning CLONING: When word games kill. An exploration by Dianne N. Irving, Ph.D. PROBLEMS with President Clinton's bioethics committee (the NBAC). RICH TEACHINGS on bioethical issues. DEFINITION of "cloning" is part of the problem. The ABAC objected strenuously. TRANSCRIPTS of NBAC cloning hearings (1.2M zip file). You won't find these on their official web site. ABAC report: Ban Human Cloning ALERT on confusion within the presidential Commission's report. CLINTON administration opposes an international ban on human cloning. NBAC continues to study other issues. CONGRESSIONAL hearing on Those who testified included Dr. Dianne Irving Rep. Vernon Ehlers Cardinal William Keeler Nigel Cameron and Fr. Kevin Fitzgerald Other cloning articles: GENETICIST Jerome Lejeune's observations about cloning. DONUM VITAE is the Catholic Church's response to cloning and similar abuses of human sexuality. LUTHERAN theologian Gilbert Meilaender on cloning OUTLAW CLONING!

149. CNN.com - Sen. Thurmond Backs Cloning Research - May 3, 2002 CNN http://cnn.com/2002/ALLPOLITICS/05/03/thurmond.cloning/index.html

MAIN PAGE WORLD U.S. WEATHER ... ABOUT US/HELP CNN TV what's on show transcripts CNN Headline News CNN International ... askCNN EDITIONS CNN.com Asia CNN.com Europe CNNenEspanol.com CNNArabic.com ... set your edition Languages Spanish Portuguese German Italian Korean Arabic Japanese Time, Inc. Time.com People Fortune EW InStyle Business 2.0 Sen. Thurmond backs cloning research From Dana Bash CNN Washington WASHINGTON (CNN) Sen. Strom Thurmond became the second Republican opponent of abortion rights this week to split with the Bush administration, signing onto a bill that would ban most human cloning but allow an exception for research purposes. The 99-year-old senator followed the lead of conservative Republican Sen. Orrin Hatch of Utah, who threw his support behind the legislation earlier this week. "During my consideration of the new and emerging areas of regenerative medicine, including nuclear transplantation technologies, two basic principles have guided my thoughts," said Thurmond, R-South Carolina, in a written statement. "First, as someone who has taken a pro-life stance, I believe that Congress should pursue policies that encourage the development of life-saving treatments. Second, nuclear transplantation research, if performed under the strictest of safeguards, is both moral and ethical." The bill sponsored by Sen. Edward Kennedy, D-Massachusetts, Sen. Dianne Feinstein, D-California, and Sen. Arlen Specter, R-Pennsylvania would make human cloning a federal crime, with penalties up to $1 million dollars and 10 years in prison. But it would allow research cloning, or what scientists and advocates refer to as "regenerative medicine," which they say has the potential to find cures for diseases such as Parkinson's, Alzheimer's and diabetes.

150. PCStats.com - Get The 'Stats And Stay Informed! GeIL. TwinMOS. KingMax. Total Votes 14602. The Rydium NetworkRydium Network. Beginners Guides cloning WindowsXP. Advertisement. Beginners Guides cloning WindowsXP. http://www.pcstats.com/articleview.cfm?articleID=418

151. Wesley J. Smith On Cloning On National Review Online Prolife perspective on human cloning by Wesley J. Smith from National Review Online http://www.nationalreview.com/comment/comment-smith011402.shtml

BACK TO NRO Close the Door on Cloning Cloning advocates try fooling Americans. By Wesley J. Smith, a frequent contributor to NRO and author of Culture of Death: The Assault on Medical Ethics in America January 14, 2002 8:25 a.m. here's an old saw about a man whose wife comes home unexpectedly and finds him in bed with his naked mistress. "Who is that woman?" the outraged wife demands. The man, a surprised and innocent look on his face, says: "Woman? What woman?" Cloning apologists remind me of that philandering husband. Their opponents point out that a cloned human embryo is a human life, and the cloners reply with: "Human life? What human life?" Unfortunately, it seems to be working, as the media and nervous politicians continue parroting the line that a human-clone embryo is not really human. The biotech industry has nothing to lose and everything to gain from this. Hoping to make vast fortunes from patented "products" derived from the destruction of embryonic life, Big Biotech is counting on being able to create an unlimited supply of human clones. Their problem: The American people believe there is something inherently valuable about human life. Cloning sheep and other animals is one thing — but cloning humans, that's different.

152. CNN - Should We Be Cloning Around? - Feb. 24, 1997 Should we be cloning around? Due to ethical concerns, Britain has banned human cloning, and research using human embryos is strictly regulated. http://www.cnn.com/TECH/9702/24/cloned.sheep/

Should we be cloning around? Breakthrough raises exciting and scary possibilities February 24, 1997 Web posted at: 3:45 p.m. EST (CNN) The announcement that a team of British scientists had successfully cloned an adult sheep has touched off a new wave of discussion over the ethical implications of such a feat. The achievement announced Sunday by a team of scientists at the Roslin Institute near Edinburgh, Scotland , marks the first time anyone has successfully cloned an adult mammal. "There are a number of genetic diseases for which there is no cure ... and this will enable us to carry out research into the causes of those diseases and perhaps develop method to treat them," Dr. Ian Wilmut of the Roslin Institute said following the announcement. While some scientists hail the cloning as a major breakthrough for research in agriculture, aging, medicine and genetics, others worry what it may portend. If sheep can be replicated, they ask, are humans far behind? Suddenly the stuff of science fiction doesn't seem so fanciful anymore as one considers the possibility of dictators cloning themselves, dead geniuses brought back to life, or beloved family pets resurrected. Sheep, cattle, pigs ... what next?

153. Human Reproductive Cloning From The Perspective Of The Future Human Reproductive cloning from the Perspective of the Future. And imagine yourself listening in to the current arguments for making cloning illegal. http://www.nickbostrom.com/views/cloning.html

Human Reproductive Cloning from the Perspective of the Future Nick Bostrom Faculty of Philosophy, Oxford University Chair, World Transhumanist Association 27 December 2002 [Slightly revised 5 April 2004] Imagine that you are one of the human clones that will be born (there is little doubt that this will happen sooner or later). And imagine yourself listening in to the current arguments for making cloning illegal. You hear people opining that cloning threatens human dignity, that it would be playing God, that it represents a slippery slope towards a dehumanized future, that everybody has a right to a unique genome (except identical twins?) or to an unknown genome, and so forth. How would it make you feel? To hear all these dignified people talking about you as if your very existence were a crime against humanity? Such an imaginary point-of-view can help us put things in perspective. There is one argument that, as a future clone, you might understand and agree with: concerns about the safety of the procedure. The argument that we ought to postpone human cloning until we have perfected the method in animals makes some degree of sense. (Even so, suppose you were a slightly deformed human clone - would you agree that it was a terrible moral offense to have caused you to come into existence?) Historically, we find that many a great medical breakthrough, now rightly seen as a blessing, was in its own time condemned by bioconservative moralists. This was the case with anesthesia during surgery and childbirth. People argued that it was unnatural and that it would weaken our moral fiber. It was also the case with heart transplantations. How yucky to take a living heart out of one person and put it in the chest of another! And it was the case with

154. Applied Genetics News: CLONING: Return To The Future Cells from six healthy cow clones show no signs of the premature aging reported for Dolly the cloned sheep, according to researchers from Advanced Cell Technologies, Inc. http://www.findarticles.com/m0DED/10_20/62404373/p1/article.jhtml

@import url(/css/us/style.css); @import url(/css/us/searchResult1.css); @import url(/css/us/articles.css); Advanced Search Home Help IN all publications this publication Reference Automotive Business Computing Entertainment Health News Reference Sports YOU ARE HERE Articles Applied Genetics News May, 2000 Content provided in partnership with Print friendly Tell a friend Find subscription deals CLONING: Return to the Future Applied Genetics News May, 2000 Cells from six healthy cow clones show no signs of the premature aging reported for Dolly the cloned sheep, according to researchers from Advanced Cell Technologies, Inc. (One Innovation Dr., Worcester, MA 01650; Tel: 508/756-1212, Fax: 508/756-0931; Website: www.advancedcell.com). To the contrary, the cloning process seems to have sent the cow cells' aging backward, making them appear even younger than cells from normal cows of the same age. To create the cow clones, the researchers used cells that were near the end of their life span in terms of division cycles, with only a few rounds of cell division left. Surprisingly, they discovered that the cloning process seemed to restore the division potential of these cells in the six cows. Instead of being 0-4 division cycles away from the end of their lives, cells taken from the cows were more than 90 cycles away from their end. Shorter telomeres are often found in older cells. Scientists spotted this telltale sign of maturity in Dolly, the original cloned sheep. Telomeres from the cloned cows, however, are actually longer than those from normal cows of the same age, and in most cases even longer than those from newborn calves.

155. Project Idaho - Home Page Woods believes the breakthrough understanding of cellular biology necessary for horse cloning to proceed may offer new insights into cancer development in http://www.uidaho.edu/cloning/

UI Home Contact UI Search news releases ... FAQ University of Idaho University Communications P.O. Box 443221 Moscow, ID 83844-3221 May 29, 2003 University of Idaho, Utah State University Team First to Successfully Clone Equine A University of Idaho-Utah State University research team is the first worldwide to clone a member of the horse family, a mule, according to an article to be published in the Journal of Science. The research team includes Gordon Woods, UI professor of animal and veterinary science, Kenneth L. White, Utah State University professor of animal science, and Dirk Vanderwall, UI assistant professor of animal and veterinary science. The baby mule, Idaho Gem, was born May 4. It is the first clone of a hybrid animal. A mule results from a cross between a female horse, a mare, and a male donkey, a jack. As hybrids, mules are sterile, except in extremely rare cases.

156. Cloning, Are Humans Next? Discusses what cloning is, how cloning could be used, whether it is right or wrong, early attempts at cloning, and Dolly, the cloned sheep; from World Book Encyclopedia. http://www2.worldbook.com/features/features.asp?feature=cloning&page=html/cl

157. Cloning The Brave New World of Mammalian cloning. The demonstration by Wilmut et al. that reprogrammed. Applications of cloning Technology. http://www.ucalgary.ca/UofC/eduweb/virtualembryo/cloning.html

All traffic to The Virtual Embryo has been redirected to its new home. If you have bookmarked this page, please update your bookmark to this location. CONTENTS Main Page Dynamic Development The Foundations of Developmental Biology Gametogenesis From Sperm and Egg to Embryo ... Developmental Biology Tutorial The Brave New World of Mammalian Cloning The demonstration by Wilmut et al. that a nucleus from an adult mammary gland cell from a sheep can be reprogrammed by the oocyte cytoplasm to direct the development of a new individual has caused developmental biologists to re-examine the process of cell differentiation. This process does not, as we have thought until quite recently, irreversibly preclude totipotency. Clearly, cytoplasmic factors are capable of evoking the entire developmental process from the nucleus of a differentiated adult cell. Since the initial report of cloned sheep, other investigators have reported bovine cloning. More recently, calves have been cloned that have been genetically modified to produce pharmaceuticals: a step toward "pharming". In a recent development, Wakayama

158. Free Online Book: The Genetic Revolution, Human Genetics, Human Cloning, Genetic Topics discussed include human cloning, genetic engineering, designer people, gene therapy, germ warfare, genetic screening, genetically modified animals and plants, superbreeds, laws, regulations and ethics / morality debate. http://www.globalchange.com/books/Genesintro.htm

Search over 26,414 pages on the Future Home Site Intro (Movie) Mailing List - News Human Cloning ... Trends Analysis by Dr Patrick Dixon Futurist Recent Video / Articles by Dr Patrick Dixon 5.5 million hits in 12 months 4 million visitors on this site THE GENETIC REVOLUTION Book by Dr Patrick Dixon c 1995 - publ Kingsway. For many updates and RealVideo comments on issues raised / latest news press here 40 videos on cloning etc 'Secret research on Embryos' - Sunday Times. 'Has Britain really made human clones' - Mail on Sunday. 'Doctor's warning over Super Veg' - Scotsman. 'Grim genes warning' - Christian Herald. 'New call to halt march of genetic engineering' - Universe. 'Human pigs horror' - News of the World INSTANT ROBOT TRANSLATOR SURF IN DIFFERENT LANGUAGES Creates a new version of the book: French German, Spanish, Italian, Chinese, Japanese and Korean Takes a few seconds - can be amusing - not 100% accurate, but remarkable

159. Cac.psu.edu/~gsg109/qs/ cloning Q A Biblical and scientific information on cloning cloning Questions and Answers. What is cloning? Is it Biblically right or wrong, and how is Genesis creation relevant? cloning Right or Wrong? http://cac.psu.edu/~gsg109/qs/

160. AFLP Not Only For Fingerprinting, But For Positional Cloning Introduction and detailed protocol by M Liscum and P Oeller, Department of Plant Biology, Carnegie Institution Stanford University, USA. http://www-ciwdpb.stanford.edu/publications/methods/aflp.html

AFLP: not only for fingerprinting, but for positional cloning Mannie Liscum(1) and Paul Oeller(2) Department of Plant Biology Carnegie Institution of Washington Stanford, CA 94305 (1)Current address: http://www.biosci.missouri.edu/liscum/LiscumLabPage.html (site contains updated information) Division of Biological Sciences 105 Tucker Hall University of Missouri Columbia, MO 65211 phone: 573-882-2672 fax: 573-882-0123 email: mliscum@biosci.mbp.missouri.edu (2)Current address: DNA Plant Technology Corporation 6701 San Pablo Avenue Oakland, CA 94609 phone: 510-547-2395 fax: 510-547-2817 email: oeller@dnap.com NOTE: The protocol presented below is based on the Amplified Fragment Length Polymorphism (AFLP) technology developed by Marc Zabeau and colleagues at Keygene N.V., Agrobusiness Park 90, P.O. Box 216, NL-6700 AE Wageningen, Netherlands (Zabeau, 1992; Zabeau and Vos, 1993; Vos et al., 1995). The AFLP technology is covered by patents and patent applications owned by Keygene N.V. Both, Life Technologies (Gathersberg, MD, USA) and Perkin Elmer (Applied Biosystems Division, Foster City, CA, USA) market research kits (under license) for AFLP fingerprinting of plant DNAs. Background Rational AFLP Protocol (Abridged Version 1.3, 12/95)

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